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gap43 recombinant protein  (Novus Biologicals)


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    Structured Review

    Novus Biologicals gap43 recombinant protein
    Gap43 Recombinant Protein, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+43+gap43/Recombinant+Human+GAP-43+His+Protein/pm41337934-88-19-22
    Average 93 stars, based on 1 article reviews
    gap43 recombinant protein - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Transplantation of MiR-28-5p-Modified BMSCs Promotes Functional Recovery After Spinal Cord Injury.
    Article Snippet: The initial blocking step involved incubating the samples with 10% FBS (Gibco, Life Technologies, USA) for 1 h at RT. .. Subsequently, tissues or cells were subjected to overnight incubation at 4 °C with primary antibodies, including microtubule-associated protein 2 (MAP2) at a dilution of 1:200 (Boster Biological Engineering Co.), neuron-specific enolase (NSE) at a dilution of 1:200 (Millipore), β3-tubulin at a dilution of 1:200 (Boster Biological Engineering Co.), neurofilament 200 (NF-200) at a dilution of 1:400 (CST), Notch1 at a dilution of 1:200 (Abcam), glial fibrillary acidic protein (GFAP) at a dilution of 1:600 (Boster Biological Engineering Co.), growth-associated protein 43 (GAP43) at a dilution of 1:200 (NOVUS), and myelin basic protein (MBP) at a dilution of 1:200 (Boster Biological Engineering Co.). .. The following day, secondary antibodies conjugated with Alexa Fluor® fluorochrome (at a dilution of 1:300, Invitrogen) were utilized to detect the respective primary antibodies, while the cell nuclei were counterstained with DAPI.

    Article Title: Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy
    Article Snippet: For the IHC analysis, the sections were fixed with 4% paraformaldehyde (Sigma, St. Louis, MO, USA) for 15 minutes and blocked for another 15 minutes. .. The following primary antibodies were then added and incubated for 2 hours at room temperature: mouse monoclonal antibody against cellular fibronectin (MAB-1940; Millipore, Billerica, MA, USA) diluted 1∶100, tenascin-C (SC-20932; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1∶200, mouse monoclonal antibody against heat shock protein 47 (HSP47) (ADI-SPA-470; Enzo Life Sciences, Lausen, Switzerland) diluted 1:200, rabbit polyclonal antibody against CD11b (AB-133357; Abcam, Cambridge, UK) diluted 1:100, mouse monoclonal antibody against α–smooth muscle actin (α-SMA) (M0851; Dako, Glostrup, Denmark) diluted 1:100, mouse monoclonal antibody against CD45 (Ab-10558; Abcam) diluted 1:100, mouse monoclonal antibody against CD31 (Ab-199012; Abcam) diluted 1:100, mouse monoclonal antibody against CD90 (LS-B3139; LifeSpan Biosciences, CA, USA) diluted 1:100 and growth-associated protein-43 (GAP43) (NB300-143; Novus Biologicals, Littleton, CO, USA) diluted 1∶500, telomerase (MA5-16034, Thermofisher, Waltham, MA, USA) diluted 1:100, and P63 (Sc8431; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1:50. .. After washing with 1× phospahte-buffered saline, the sections were incubated with goat anti-mouse Alexa Fluor 488–conjugated secondary antibody or goat anti-rabbit Alexa Fluor 594–conjugated secondary antibody (Invitrogen, Carlsbad, CA, United States) at room temperature for 1 hour.

    Article Title: Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Olfactory Sensory Neurons Regulate Axon Extension and Glomerular Formation
    Article Snippet: Serial sections (20 m thick) of the OE and OB were collected on Superfrost Plus slides (VWR) and stored at 20°C until used. .. Slides were heated to 37°C for 10 min, blocked in wash buffer (0.1 M PBS, 2% BSA, 0.3% Triton X-100) for 30 min, and incubated in the following primary antibodies at 4°C overnight; chicken antibody: anti-MAP-2 at 1:1000 (Millipore); rabbit antibodies: ACIII at 1:250 (Santa Cruz Biotechnology), growthassociated protein 43 (GAP43) at 1:1000 (Novus), HCN1 at 1:250, peripherin at 1:2000 (Millipore), olfactory cell adhesion molecule (OCAM) 1:500 (generous gift from Dr. Yoshihiro Yoshihara, RIKEN Brain Science Institute, Wako, Japan), and vesicular glutamate transporter 2 (VGlut2) at 1:2000 (Synaptic Systems); rat antibodies: HCN4 at 1:500 (Abcam), HCN4 at 1:1000 (Alomone) and NCAM at 1:1000 (Millipore); goat antibodies: olfactory marker protein (OMP) at 1:1000 (Wako Pure Chemical Industries) and neuropilin 1 (Nrp1) at 1:750 (R&D Systems); guinea pig anti-HCN2 at 1:1000 (generous gift from Dr. Ryuichi Shigemoto); rabbit anti-HCN2 (HCN2 ); and guinea pig antiHCN4 (HCN4 ) (F.M.). .. After rinsing in wash buffer, slides were incubated with fluorescent secondary antibodies (donkey anti-rabbit Alexa Fluor 488 or Alexa Fluor 555; donkey anti-rat Alexa 594; donkey anti-goat Alexa Fluor 488 or Alexa Fluor 555; donkey anti-guinea pig Alexa 488; all used at 1:1000 and all from Invitrogen) and nuclear stain DRAQ5 at 1:1000 (Biostatus) for 1 h at room temperature, rinsed and coverslipped with BioMeda GelMount (Electron Microscopy Sciences).

    Article Title: Transplantation of MiR-28-5p-Modified BMSCs Promotes Functional Recovery After Spinal Cord Injury
    Article Snippet: The initial blocking step involved incubating the samples with 10% FBS (Gibco, Life Technologies, USA) for 1 h at RT. .. Subsequently, tissues or cells were subjected to overnight incubation at 4 °C with primary antibodies, including microtubule-associated protein 2 (MAP2) at a dilution of 1:200 (Boster Biological Engineering Co.), neuron-specific enolase (NSE) at a dilution of 1:200 (Millipore), β3-tubulin at a dilution of 1:200 (Boster Biological Engineering Co.), neurofilament 200 (NF-200) at a dilution of 1:400 (CST), Notch1 at a dilution of 1:200 (Abcam), glial fibrillary acidic protein (GFAP) at a dilution of 1:600 (Boster Biological Engineering Co.), growth-associated protein 43 (GAP43) at a dilution of 1:200 (NOVUS), and myelin basic protein (MBP) at a dilution of 1:200 (Boster Biological Engineering Co.). .. The following day, secondary antibodies conjugated with Alexa Fluor® fluorochrome (at a dilution of 1:300, Invitrogen) were utilized to detect the respective primary antibodies, while the cell nuclei were counterstained with DAPI.

    Marker:

    Article Title: Fas-Associated Factor 1 as a Regulator of Olfactory Axon Guidance
    Article Snippet: .. Primary antibodies used were anti-growth-associated protein 43 (GAP43) (1:1000, Novus Biologicals), anti-olfactory marker protein (OMP) (1:5000, Wako Chemicals), anti-FAF1 (1:1000, Protein Tech Group), anti-cleaved caspase-3 (1:1000, Cell Signaling Technology), anti- -galactosidase ( -gal) (1:1000, ICN Biomedicals). .. Cy3-conjugated secondary antibodies were used at a 1:1000 dilution (Jackson ImmunoResearch).

    Article Title: Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Olfactory Sensory Neurons Regulate Axon Extension and Glomerular Formation
    Article Snippet: Serial sections (20 m thick) of the OE and OB were collected on Superfrost Plus slides (VWR) and stored at 20°C until used. .. Slides were heated to 37°C for 10 min, blocked in wash buffer (0.1 M PBS, 2% BSA, 0.3% Triton X-100) for 30 min, and incubated in the following primary antibodies at 4°C overnight; chicken antibody: anti-MAP-2 at 1:1000 (Millipore); rabbit antibodies: ACIII at 1:250 (Santa Cruz Biotechnology), growthassociated protein 43 (GAP43) at 1:1000 (Novus), HCN1 at 1:250, peripherin at 1:2000 (Millipore), olfactory cell adhesion molecule (OCAM) 1:500 (generous gift from Dr. Yoshihiro Yoshihara, RIKEN Brain Science Institute, Wako, Japan), and vesicular glutamate transporter 2 (VGlut2) at 1:2000 (Synaptic Systems); rat antibodies: HCN4 at 1:500 (Abcam), HCN4 at 1:1000 (Alomone) and NCAM at 1:1000 (Millipore); goat antibodies: olfactory marker protein (OMP) at 1:1000 (Wako Pure Chemical Industries) and neuropilin 1 (Nrp1) at 1:750 (R&D Systems); guinea pig anti-HCN2 at 1:1000 (generous gift from Dr. Ryuichi Shigemoto); rabbit anti-HCN2 (HCN2 ); and guinea pig antiHCN4 (HCN4 ) (F.M.). .. After rinsing in wash buffer, slides were incubated with fluorescent secondary antibodies (donkey anti-rabbit Alexa Fluor 488 or Alexa Fluor 555; donkey anti-rat Alexa 594; donkey anti-goat Alexa Fluor 488 or Alexa Fluor 555; donkey anti-guinea pig Alexa 488; all used at 1:1000 and all from Invitrogen) and nuclear stain DRAQ5 at 1:1000 (Biostatus) for 1 h at room temperature, rinsed and coverslipped with BioMeda GelMount (Electron Microscopy Sciences).

    Olfactory:

    Article Title: Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Olfactory Sensory Neurons Regulate Axon Extension and Glomerular Formation
    Article Snippet: Serial sections (20 m thick) of the OE and OB were collected on Superfrost Plus slides (VWR) and stored at 20°C until used. .. Slides were heated to 37°C for 10 min, blocked in wash buffer (0.1 M PBS, 2% BSA, 0.3% Triton X-100) for 30 min, and incubated in the following primary antibodies at 4°C overnight; chicken antibody: anti-MAP-2 at 1:1000 (Millipore); rabbit antibodies: ACIII at 1:250 (Santa Cruz Biotechnology), growthassociated protein 43 (GAP43) at 1:1000 (Novus), HCN1 at 1:250, peripherin at 1:2000 (Millipore), olfactory cell adhesion molecule (OCAM) 1:500 (generous gift from Dr. Yoshihiro Yoshihara, RIKEN Brain Science Institute, Wako, Japan), and vesicular glutamate transporter 2 (VGlut2) at 1:2000 (Synaptic Systems); rat antibodies: HCN4 at 1:500 (Abcam), HCN4 at 1:1000 (Alomone) and NCAM at 1:1000 (Millipore); goat antibodies: olfactory marker protein (OMP) at 1:1000 (Wako Pure Chemical Industries) and neuropilin 1 (Nrp1) at 1:750 (R&D Systems); guinea pig anti-HCN2 at 1:1000 (generous gift from Dr. Ryuichi Shigemoto); rabbit anti-HCN2 (HCN2 ); and guinea pig antiHCN4 (HCN4 ) (F.M.). .. After rinsing in wash buffer, slides were incubated with fluorescent secondary antibodies (donkey anti-rabbit Alexa Fluor 488 or Alexa Fluor 555; donkey anti-rat Alexa 594; donkey anti-goat Alexa Fluor 488 or Alexa Fluor 555; donkey anti-guinea pig Alexa 488; all used at 1:1000 and all from Invitrogen) and nuclear stain DRAQ5 at 1:1000 (Biostatus) for 1 h at room temperature, rinsed and coverslipped with BioMeda GelMount (Electron Microscopy Sciences).

    Membrane:

    Article Title: Transplantation of Wnt5a-modified Bone Marrow Mesenchymal Stem Cells Promotes Recovery After Spinal Cord Injury via the PI3K/AKT Pathway
    Article Snippet: .. The membrane was sealed using pre-chilled NcmBlot Rapid Closure Solution, and primary antibodies were added at 4°C: microtubule-associated protein-2 (MAP-2) (1:1000; Boster Biological Engineering Co.), glial fibrillary acidic protein (GFAP) (1:1000; Boster Biological Engineering Co.), β3-tubulin (1:1000; CST), growth-associated protein 43 (GAP43) (1:1000; NOVUS), myelin basic protein (MBP) (1:1000; NOVUS), PI3K (1:1000; CST, 4228), p-PI3K (1:1000; Bioss), AKT (1:1000; CST), p-AKT (1:1000; CST), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:500; Thermo Fisher). .. After rinsing the membranes with Tris-buffered saline with Tween (TBST), they were incubated for 60 minutes with a secondary antibody (1:1000; Boster Biological Engineering Co.).

    Article Title: Transplantation of Wnt5a-modified Bone Marrow Mesenchymal Stem Cells Promotes Recovery After Spinal Cord Injury via the PI3K/AKT Pathway.
    Article Snippet: .. The membrane was sealed using pre-chilled NcmBlot Rapid Closure Solution, and primary antibodies were added at 4°C: microtubule-associated protein-2 (MAP-2) (1:1000; Boster Biological Engineering Co.), glial fibrillary acidic protein (GFAP) (1:1000; Boster Biological Engineering Co.), β3-tubulin (1:1000; CST), growthassociated protein 43 (GAP43) (1:1000; NOVUS), myelin basic protein (MBP) (1:1000; NOVUS), PI3K (1:1000; CST, 4228), p-PI3K (1:1000; Bioss), AKT (1:1000; CST), p-AKT (1:1000; CST), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:500; Thermo Fisher). .. After rinsing the membranes with Tris-buffered saline with Tween (TBST), they were incubated for 60 minutes with a secondary antibody (1:1000; Boster Biological Engineering Co.).



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    Image Search Results


    Fig. 4. Modulation of neurohormonal activity by stellate ganglionectomy in chronic heart failure. (A) Western blotting was used to detect the protein expression levels of tyrosine hydroxylase (TH) and growth-associated protein-43 (GAP43) in the cardiac tissues of

    Journal: Frontiers in Bioscience-Landmark

    Article Title: Stellate Ganglionectomy Attenuates Pressure Overload-Induced Cardiac Hypertrophy and Dysfunction

    doi: 10.31083/fbl26268

    Figure Lengend Snippet: Fig. 4. Modulation of neurohormonal activity by stellate ganglionectomy in chronic heart failure. (A) Western blotting was used to detect the protein expression levels of tyrosine hydroxylase (TH) and growth-associated protein-43 (GAP43) in the cardiac tissues of

    Article Snippet: Then, the membranes were incubated with primary antibodies against atrial natriuretic peptides (ANP) (1:500, PL0403306, PL Laboratories, Beijing, China), growthassociated protein-43 (GAP43) (1:500, 16971-1-AP, proteintech, Rosemont, IL, USA), Tyrosine Hydroxylase (TH) (1:500, 250905, Abbiotec, Danvers, MA, USA), CaMKII (1:5000, BW6769, Abcam, Cambridge, MA, USA), Ryanodine Receptor 2 (RyR2, 1:500, orb794741, Biorbyt, UK), phosphorylated (p) -RyR2 (1:500, BS4358, Bioworld, Danvers, MA ,USA).

    Techniques: Activity Assay, Western Blot, Expressing