gap43 recombinant protein (Novus Biologicals)
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Gap43 Recombinant Protein, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+43+gap43/Recombinant+Human+GAP-43+His+Protein/pm41337934-88-19-22
Average 93 stars, based on 1 article reviews
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Incubation:Article Title: Transplantation of MiR-28-5p-Modified BMSCs Promotes Functional Recovery After Spinal Cord Injury. Article Snippet: The initial blocking step involved incubating the samples with 10% FBS (Gibco, Life Technologies, USA) for 1 h at RT. .. Subsequently, tissues or cells were subjected to overnight incubation at 4 °C with primary antibodies, including microtubule-associated protein 2 (MAP2) at a dilution of 1:200 (Boster Biological Engineering Co.), neuron-specific enolase (NSE) at a dilution of 1:200 (Millipore), β3-tubulin at a dilution of 1:200 (Boster Biological Engineering Co.), neurofilament 200 (NF-200) at a dilution of 1:400 (CST), Notch1 at a dilution of 1:200 (Abcam), glial fibrillary acidic protein (GFAP) at a dilution of 1:600 (Boster Biological Engineering Co.), growth-associated Article Title: Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy Article Snippet: For the IHC analysis, the sections were fixed with 4% paraformaldehyde (Sigma, St. Louis, MO, USA) for 15 minutes and blocked for another 15 minutes. .. The following primary antibodies were then added and incubated for 2 hours at room temperature: mouse monoclonal antibody against cellular fibronectin (MAB-1940; Millipore, Billerica, MA, USA) diluted 1∶100, tenascin-C (SC-20932; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1∶200, mouse monoclonal antibody against heat shock protein 47 (HSP47) (ADI-SPA-470; Enzo Life Sciences, Lausen, Switzerland) diluted 1:200, rabbit polyclonal antibody against CD11b (AB-133357; Abcam, Cambridge, UK) diluted 1:100, mouse monoclonal antibody against α–smooth muscle actin (α-SMA) (M0851; Dako, Glostrup, Denmark) diluted 1:100, mouse monoclonal antibody against CD45 (Ab-10558; Abcam) diluted 1:100, mouse monoclonal antibody against CD31 (Ab-199012; Abcam) diluted 1:100, mouse monoclonal antibody against CD90 (LS-B3139; LifeSpan Biosciences, CA, USA) diluted 1:100 and growth-associated Article Title: Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Olfactory Sensory Neurons Regulate Axon Extension and Glomerular Formation Article Snippet: Serial sections (20 m thick) of the OE and OB were collected on Superfrost Plus slides (VWR) and stored at 20°C until used. .. Slides were heated to 37°C for 10 min, blocked in wash buffer (0.1 M PBS, 2% BSA, 0.3% Triton X-100) for 30 min, and incubated in the following primary antibodies at 4°C overnight; chicken antibody: anti-MAP-2 at 1:1000 (Millipore); rabbit antibodies: ACIII at 1:250 (Santa Cruz Biotechnology), growthassociated Article Title: Transplantation of MiR-28-5p-Modified BMSCs Promotes Functional Recovery After Spinal Cord Injury Article Snippet: The initial blocking step involved incubating the samples with 10% FBS (Gibco, Life Technologies, USA) for 1 h at RT. .. Subsequently, tissues or cells were subjected to overnight incubation at 4 °C with primary antibodies, including microtubule-associated protein 2 (MAP2) at a dilution of 1:200 (Boster Biological Engineering Co.), neuron-specific enolase (NSE) at a dilution of 1:200 (Millipore), β3-tubulin at a dilution of 1:200 (Boster Biological Engineering Co.), neurofilament 200 (NF-200) at a dilution of 1:400 (CST), Notch1 at a dilution of 1:200 (Abcam), glial fibrillary acidic protein (GFAP) at a dilution of 1:600 (Boster Biological Engineering Co.), growth-associated Marker:Article Title: Fas-Associated Factor 1 as a Regulator of Olfactory Axon Guidance Article Snippet: .. Primary antibodies used were anti-growth-associated Article Title: Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Olfactory Sensory Neurons Regulate Axon Extension and Glomerular Formation Article Snippet: Serial sections (20 m thick) of the OE and OB were collected on Superfrost Plus slides (VWR) and stored at 20°C until used. .. Slides were heated to 37°C for 10 min, blocked in wash buffer (0.1 M PBS, 2% BSA, 0.3% Triton X-100) for 30 min, and incubated in the following primary antibodies at 4°C overnight; chicken antibody: anti-MAP-2 at 1:1000 (Millipore); rabbit antibodies: ACIII at 1:250 (Santa Cruz Biotechnology), growthassociated Olfactory:Article Title: Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels in Olfactory Sensory Neurons Regulate Axon Extension and Glomerular Formation Article Snippet: Serial sections (20 m thick) of the OE and OB were collected on Superfrost Plus slides (VWR) and stored at 20°C until used. .. Slides were heated to 37°C for 10 min, blocked in wash buffer (0.1 M PBS, 2% BSA, 0.3% Triton X-100) for 30 min, and incubated in the following primary antibodies at 4°C overnight; chicken antibody: anti-MAP-2 at 1:1000 (Millipore); rabbit antibodies: ACIII at 1:250 (Santa Cruz Biotechnology), growthassociated Membrane:Article Title: Transplantation of Wnt5a-modified Bone Marrow Mesenchymal Stem Cells Promotes Recovery After Spinal Cord Injury via the PI3K/AKT Pathway Article Snippet: .. The membrane was sealed using pre-chilled NcmBlot Rapid Closure Solution, and primary antibodies were added at 4°C: microtubule-associated protein-2 (MAP-2) (1:1000; Boster Biological Engineering Co.), glial fibrillary acidic protein (GFAP) (1:1000; Boster Biological Engineering Co.), β3-tubulin (1:1000; CST), growth-associated Article Title: Transplantation of Wnt5a-modified Bone Marrow Mesenchymal Stem Cells Promotes Recovery After Spinal Cord Injury via the PI3K/AKT Pathway. Article Snippet: .. The membrane was sealed using pre-chilled NcmBlot Rapid Closure Solution, and primary antibodies were added at 4°C: microtubule-associated protein-2 (MAP-2) (1:1000; Boster Biological Engineering Co.), glial fibrillary acidic protein (GFAP) (1:1000; Boster Biological Engineering Co.), β3-tubulin (1:1000; CST), growthassociated |
